APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human PCSK9.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proprotein convertase subtilisin/kexin type 9 , FH3 , HCHOLA3 , LDLCQ1 , NARC-1 , NARC1 , PC9
Cellular Localization
Cytoplasm,Secreted,Endosome,Lysosome,Cell surface,Endoplasmic reticulum,Golgi apparatus
Background
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Database
Research Area
DATA IMAGES
![Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330](/templates/default/js/lightbox/images/loading.gif "Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330")
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GTX639330 WB Image
Wild-type (WT) and PCSK9 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody [HL2666] (GTX639330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX639330 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody [HL2666] (GTX639330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX639330 IHC-P Image
PCSK9 antibody [HL2666] detects secreted PCSK9 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.PCSK9 stained by PCSK9 antibody [HL2666] (GTX639330) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639330 IHC-P Image
PCSK9 antibody [HL2666] detects secreted PCSK9 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.PCSK9 stained by PCSK9 antibody [HL2666] (GTX639330) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for PCSK9 antibody [HL2666] (GTX639330). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PCSK9 antibody [HL2666] (GTX639330). Be the first to share your experience with this product.
![Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330](/upload/website/prouct_img/small/GTX639330/GTX639330_T-45215_20231103_WB_KO_watermark_23110819_252.webp "Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330")
![Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330](/upload/website/prouct_img/small/GTX639330/GTX639330_45299_20240126_WB_TPM_watermark_24013018_728.webp "Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330")
![Anti-PCSK9 antibody [HL2666] used in IHC (Paraffin sections) (IHC-P). GTX639330](/upload/website/prouct_img/small/GTX639330/GTX639330_T-45215_20231201_IHC-P_R_23120519_693.webp "Anti-PCSK9 antibody [HL2666] used in IHC (Paraffin sections) (IHC-P). GTX639330")
![Anti-PCSK9 antibody [HL2666] used in IHC (Paraffin sections) (IHC-P). GTX639330](/upload/website/prouct_img/small/GTX639330/GTX639330_T-45215_20231201_IHC-P_M_24010223_978.webp "Anti-PCSK9 antibody [HL2666] used in IHC (Paraffin sections) (IHC-P). GTX639330")
![Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330](/upload/website/prouct_img/normal/GTX639330/GTX639330_T-45215_20231103_WB_KO_watermark_23110819_252.webp "Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330")
![Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330](/upload/website/prouct_img/normal/GTX639330/GTX639330_45299_20240126_WB_TPM_watermark_24013018_728.webp "Anti-PCSK9 antibody [HL2666] used in Western Blot (WB). GTX639330")
![Anti-PCSK9 antibody [HL2666] used in IHC (Paraffin sections) (IHC-P). GTX639330](/upload/website/prouct_img/normal/GTX639330/GTX639330_T-45215_20231201_IHC-P_R_23120519_693.webp "Anti-PCSK9 antibody [HL2666] used in IHC (Paraffin sections) (IHC-P). GTX639330")
![Anti-PCSK9 antibody [HL2666] used in IHC (Paraffin sections) (IHC-P). GTX639330](/upload/website/prouct_img/normal/GTX639330/GTX639330_T-45215_20231201_IHC-P_M_24010223_978.webp "Anti-PCSK9 antibody [HL2666] used in IHC (Paraffin sections) (IHC-P). GTX639330")