Wild-type (WT) and PCSK9 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody [HL2666] (GTX639330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody [HL2666] (GTX639330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

PCSK9 antibody [HL2666] detects secreted PCSK9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
PCSK9 stained by PCSK9 antibody [HL2666] (GTX639330) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PCSK9 antibody [HL2666] detects secreted PCSK9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
PCSK9 stained by PCSK9 antibody [HL2666] (GTX639330) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min