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PCSK9 antibody

KO/KD
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859

Cat No. GTX129859

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all PCSK9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCSK9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proprotein convertase subtilisin/kexin type 9 , FH3 , HCHOLA3 , LDLCQ1 , NARC-1 , NARC1 , PC9

Cellular Localization

Secreted

Background

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

KO/KD

Wild-type (WT) and PCSK9 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

PCSK9 antibody detects PCSK9 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at a dilution of 1:500.

Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859

GTX129859 FACS Image

PCSK9 antibody (GTX129859) detects PCSK9 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: PCSK9 antibody (GTX129859) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

REFERENCE

Application Reference

Chang HY et al. Molecules 2019; 24 (3) The Cholesterol-Modulating Effect of Methanol Extract of Pigeon Pea (Cajanus cajan (L.) Millsp.) Leaves on Regulating LDLR and PCSK9 Expression in HepG2 Cells.
Gao WY et al. J Agric Food Chem 2018; 66 (24) : 6083-6093 Pinostrobin Inhibits Proprotein Convertase Subtilisin/Kexin-type 9 (PCSK9) Gene Expression through the Modulation of FoxO3a Protein in HepG2 Cells.
Application : WB
Reactivity : Human
Chen SF et al. J Agric Food Chem 2017; (Epub) Xanthohumol suppresses Mylip/Idol gene expression and modulates LDLR abundance and activity in HepG2 cells.
Application : WB
Reactivity : Human
Zanoni C et al. J Funct Foods 2017; 32 : 43108 Investigations on the hypocholesterolaemic activity of LILPKHSDAD and LTFPGSAED, two peptides from lupin β-conglutin: Focus on LDLR and PCSK9 pathways.
Chen HC et al. PLoS One 2016; 11 (9) : e0162414 Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells.
Application : WB
Reactivity : Human
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Application Reference
Chang HY et al. Molecules 2019; 24 (3) The Cholesterol-Modulating Effect of Methanol Extract of Pigeon Pea (Cajanus cajan (L.) Millsp.) Leaves on Regulating LDLR and PCSK9 Expression in HepG2 Cells.
Gao WY et al. J Agric Food Chem 2018; 66 (24):6083-6093 Pinostrobin Inhibits Proprotein Convertase Subtilisin/Kexin-type 9 (PCSK9) Gene Expression through the Modulation of FoxO3a Protein in HepG2 Cells.
Application : WB
Reactivity : Human
Chen SF et al. J Agric Food Chem 2017; (Epub) Xanthohumol suppresses Mylip/Idol gene expression and modulates LDLR abundance and activity in HepG2 cells.
Application : WB
Reactivity : Human
Zanoni C et al. J Funct Foods 2017; 32:43108 Investigations on the hypocholesterolaemic activity of LILPKHSDAD and LTFPGSAED, two peptides from lupin β-conglutin: Focus on LDLR and PCSK9 pathways.
Chen HC et al. PLoS One 2016; 11 (9):e0162414 Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells.
Application : WB
Reactivity : Human
Lammi C et al. Nutrients 2016; 8 (7) Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells.
Application : WB, ELISA
Reactivity : Human
Lammi C et al. J Funct Foods 2016; 23:230-240 Lupin protein exerts cholesterol-lowering effects targeting PCSK9: From clinical evidences to elucidation of the in vitro molecular mechanism using HepG2 cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169