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PCSK9 antibody

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

Cat. No. GTX129859

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, FACS, ELISA

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Mouse, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCSK9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886111

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proprotein convertase subtilisin/kexin type 9 , FH3 , HCHOLA3 , LDLCQ1 , NARC-1 , NARC1 , PC9

Cellular Localization

Secreted

Background

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

Wild-type (WT) and PCSK9 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859

GTX129859 FACS Image

PCSK9 antibody (GTX129859) detects PCSK9 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: PCSK9 antibody (GTX129859) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

PCSK9 antibody detects PCSK9 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at a dilution of 1:500.

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

REVIEW

PCSK9 antibody Cat. No. GTX129859

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 16-Nov-2022
Score :
Application Tested : WB
Sample : Henle cell
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169