GeneTex

  • Country / Location Selection

United States (US)

PCSK9 antibody

KO/KD
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859

Cat. No. GTX129859

Reference 12
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS, ELISA

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all PCSK9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Mouse, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCSK9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886111

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proprotein convertase subtilisin/kexin type 9 , FH3 , HCHOLA3 , LDLCQ1 , NARC-1 , NARC1 , PC9

Cellular Localization

Secreted

Background

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

KO/KD

Wild-type (WT) and PCSK9 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

PCSK9 antibody detects PCSK9 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at a dilution of 1:500.

Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859

GTX129859 FACS Image

PCSK9 antibody (GTX129859) detects PCSK9 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: PCSK9 antibody (GTX129859) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

Application Reference

Stefania Sut et al. Molecules 2022; 27 (7) NMR, LC-MS Characterization of Rydingia michauxii Extracts, Identification of Natural Products Acting as Modulators of LDLR and PCSK9.
Application : WB
Reactivity : Human
Maria Giovanna Lupo et al. Int J Mol Sci 2021; 22 (8) PCSK9 Induces Rat Smooth Muscle Cell Proliferation and Counteracts the Pleiotropic Effects of Simvastatin.
Application : WB
Reactivity : Rat
Stefania Sut et al. Molecules 2021; 26 (13) The Modulation of PCSK9 and LDLR by Supercritical CO2 Extracts of Mentha longifolia and Isolated Piperitone Oxide, an In Vitro Study.
Application : WB
Reactivity : Human
Lammi C et al. Nutrients 2021; 13 (3) Trans-Epithelial Transport, Metabolism, and Biological Activity Assessment of the Multi-Target Lupin Peptide LILPKHSDAD (P5) and Its Metabolite LPKHSDAD (P5-Met).
Application : WB
Reactivity : Human
Macchi C et al. Am J Pathol 2020; 190 (11) : 2226-2236 Leptin, Resistin, and Proprotein Convertase Subtilisin/Kexin Type 9: The Role of STAT3.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

PCSK9 antibody Cat. No. GTX129859

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Date : Anonymous submitted on 16-Nov-2022
Score :
Application Tested : WB
Sample : Henle cell
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
Application Reference
Stefania Sut et al. Molecules 2022; 27 (7) NMR, LC-MS Characterization of Rydingia michauxii Extracts, Identification of Natural Products Acting as Modulators of LDLR and PCSK9.
Application : WB
Reactivity : Human
Maria Giovanna Lupo et al. Int J Mol Sci 2021; 22 (8) PCSK9 Induces Rat Smooth Muscle Cell Proliferation and Counteracts the Pleiotropic Effects of Simvastatin.
Application : WB
Reactivity : Rat
Stefania Sut et al. Molecules 2021; 26 (13) The Modulation of PCSK9 and LDLR by Supercritical CO2 Extracts of Mentha longifolia and Isolated Piperitone Oxide, an In Vitro Study.
Application : WB
Reactivity : Human
Lammi C et al. Nutrients 2021; 13 (3) Trans-Epithelial Transport, Metabolism, and Biological Activity Assessment of the Multi-Target Lupin Peptide LILPKHSDAD (P5) and Its Metabolite LPKHSDAD (P5-Met).
Application : WB
Reactivity : Human
Macchi C et al. Am J Pathol 2020; 190 (11):2226-2236 Leptin, Resistin, and Proprotein Convertase Subtilisin/Kexin Type 9: The Role of STAT3.
Application : WB
Reactivity : Human
Chang HY et al. Molecules 2019; 24 (3) The Cholesterol-Modulating Effect of Methanol Extract of Pigeon Pea (Cajanus cajan (L.) Millsp.) Leaves on Regulating LDLR and PCSK9 Expression in HepG2 Cells.
Gao WY et al. J Agric Food Chem 2018; 66 (24):6083-6093 Pinostrobin Inhibits Proprotein Convertase Subtilisin/Kexin-type 9 (PCSK9) Gene Expression through the Modulation of FoxO3a Protein in HepG2 Cells.
Application : WB
Reactivity : Human
Chen SF et al. J Agric Food Chem 2017; (Epub) Xanthohumol suppresses Mylip/Idol gene expression and modulates LDLR abundance and activity in HepG2 cells.
Application : WB
Reactivity : Human
Zanoni C et al. J Funct Foods 2017; 32:43108 Investigations on the hypocholesterolaemic activity of LILPKHSDAD and LTFPGSAED, two peptides from lupin β-conglutin: Focus on LDLR and PCSK9 pathways.
Chen HC et al. PLoS One 2016; 11 (9):e0162414 Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells.
Application : WB
Reactivity : Human
Lammi C et al. Nutrients 2016; 8 (7) Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells.
Application : WB, ELISA
Reactivity : Human
Lammi C et al. J Funct Foods 2016; 23:230-240 Lupin protein exerts cholesterol-lowering effects targeting PCSK9: From clinical evidences to elucidation of the in vitro molecular mechanism using HepG2 cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production