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PCSK9 antibody

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859

Cat. No. GTX129859

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, FACS, ELISA

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Mouse, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCSK9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886111

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proprotein convertase subtilisin/kexin type 9 , FH3 , HCHOLA3 , LDLCQ1 , NARC-1 , NARC1 , PC9

Cellular Localization

Secreted

Background

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

Wild-type (WT) and PCSK9 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

PCSK9 antibody detects PCSK9 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at a dilution of 1:500.

Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859

GTX129859 FACS Image

PCSK9 antibody (GTX129859) detects PCSK9 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: PCSK9 antibody (GTX129859) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

REVIEW

PCSK9 antibody Cat. No. GTX129859

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 16-Nov-2022
Score :
Application Tested : WB
Sample : Henle cell
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production