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PCSK9 antibody

KO/KD Orthogonal
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

Cat. No. GTX129859

Reference 13
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS, ELISA

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all PCSK9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Mouse, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCSK9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886111

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proprotein convertase subtilisin/kexin type 9 , FH3 , HCHOLA3 , LDLCQ1 , NARC-1 , NARC1 , PC9

Cellular Localization

Secreted

Background

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

KO/KD

Wild-type (WT) and PCSK9 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCSK9 antibody used in Flow cytometry (FACS). GTX129859

GTX129859 FACS Image

PCSK9 antibody (GTX129859) detects PCSK9 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: PCSK9 antibody (GTX129859) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

PCSK9 antibody detects PCSK9 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at a dilution of 1:500.

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PCSK9 antibody used in Western Blot (WB). GTX129859

GTX129859 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PCSK9 antibody (GTX129859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Panighel G et al. Food Chem (Oxf) 2022; 5 : 100126 Investigating the <i>in vitro</i> mode of action of okra (<i>Abelmoschus esculentus</i>) as hypocholesterolemic, anti-inflammatory, and antioxidant food.
Stefania Sut et al. Molecules 2022; 27 (7) NMR, LC-MS Characterization of Rydingia michauxii Extracts, Identification of Natural Products Acting as Modulators of LDLR and PCSK9.
Application : WB
Reactivity : Human
Maria Giovanna Lupo et al. Int J Mol Sci 2021; 22 (8) PCSK9 Induces Rat Smooth Muscle Cell Proliferation and Counteracts the Pleiotropic Effects of Simvastatin.
Application : WB
Reactivity : Rat
Stefania Sut et al. Molecules 2021; 26 (13) The Modulation of PCSK9 and LDLR by Supercritical CO2 Extracts of Mentha longifolia and Isolated Piperitone Oxide, an In Vitro Study.
Application : WB
Reactivity : Human
Lammi C et al. Nutrients 2021; 13 (3) Trans-Epithelial Transport, Metabolism, and Biological Activity Assessment of the Multi-Target Lupin Peptide LILPKHSDAD (P5) and Its Metabolite LPKHSDAD (P5-Met).
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

PCSK9 antibody Cat. No. GTX129859

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-PCSK9 antibody used in Western Blot (WB). GTX129859
Date : Anonymous submitted on 16-Nov-2022
Score :
Application Tested : WB
Sample : Henle cell
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
Application Reference
Panighel G et al. Food Chem (Oxf) 2022; 5:100126 Investigating the <i>in vitro</i> mode of action of okra (<i>Abelmoschus esculentus</i>) as hypocholesterolemic, anti-inflammatory, and antioxidant food.
Stefania Sut et al. Molecules 2022; 27 (7) NMR, LC-MS Characterization of Rydingia michauxii Extracts, Identification of Natural Products Acting as Modulators of LDLR and PCSK9.
Application : WB
Reactivity : Human
Maria Giovanna Lupo et al. Int J Mol Sci 2021; 22 (8) PCSK9 Induces Rat Smooth Muscle Cell Proliferation and Counteracts the Pleiotropic Effects of Simvastatin.
Application : WB
Reactivity : Rat
Stefania Sut et al. Molecules 2021; 26 (13) The Modulation of PCSK9 and LDLR by Supercritical CO2 Extracts of Mentha longifolia and Isolated Piperitone Oxide, an In Vitro Study.
Application : WB
Reactivity : Human
Lammi C et al. Nutrients 2021; 13 (3) Trans-Epithelial Transport, Metabolism, and Biological Activity Assessment of the Multi-Target Lupin Peptide LILPKHSDAD (P5) and Its Metabolite LPKHSDAD (P5-Met).
Application : WB
Reactivity : Human
Macchi C et al. Am J Pathol 2020; 190 (11):2226-2236 Leptin, Resistin, and Proprotein Convertase Subtilisin/Kexin Type 9: The Role of STAT3.
Application : WB
Reactivity : Human
Chang HY et al. Molecules 2019; 24 (3) The Cholesterol-Modulating Effect of Methanol Extract of Pigeon Pea (Cajanus cajan (L.) Millsp.) Leaves on Regulating LDLR and PCSK9 Expression in HepG2 Cells.
Gao WY et al. J Agric Food Chem 2018; 66 (24):6083-6093 Pinostrobin Inhibits Proprotein Convertase Subtilisin/Kexin-type 9 (PCSK9) Gene Expression through the Modulation of FoxO3a Protein in HepG2 Cells.
Application : WB
Reactivity : Human
Chen SF et al. J Agric Food Chem 2017; (Epub) Xanthohumol suppresses Mylip/Idol gene expression and modulates LDLR abundance and activity in HepG2 cells.
Application : WB
Reactivity : Human
Zanoni C et al. J Funct Foods 2017; 32:43108 Investigations on the hypocholesterolaemic activity of LILPKHSDAD and LTFPGSAED, two peptides from lupin β-conglutin: Focus on LDLR and PCSK9 pathways.
Chen HC et al. PLoS One 2016; 11 (9):e0162414 Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells.
Application : WB
Reactivity : Human
Lammi C et al. Nutrients 2016; 8 (7) Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells.
Application : WB, ELISA
Reactivity : Human
Lammi C et al. J Funct Foods 2016; 23:230-240 Lupin protein exerts cholesterol-lowering effects targeting PCSK9: From clinical evidences to elucidation of the in vitro molecular mechanism using HepG2 cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169