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PD-L1 antibody [10F.9G2]

Anti-PD-L1 antibody [10F.9G2] used in Western Blot (WB). GTX14145

Cat. No. GTX14145

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 10F.9G2

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-Fr, FACS, Neutralizing/Inhibition

Reactivity

 Mouse
Package
500 μg ($399)
See all PD-L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

Neutralizing/Inhibition
The 10F.9G2 antibody has been shown to block the interaction between PD-L1 and PD-1 and between PD-L1 and B7-1 (CD80).
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse CD274

Purification

Protein G purified
From tissue culture supernatant

Purity

>95% (Determined by SDS-PAGE)

Endotoxin

< 0.002 EU/弮g (Determined by LAL assay)

Conjugation

Unconjugated

RRID

AB_2887511

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PD-L1 antibody [10F.9G2] used in Western Blot (WB). GTX14145

GTX14145 WB Image

WB analysis of purified PD-L1 recombinant protein using GTX14145 PD-L1 antibody [10F.9G2].
Lane 1 : 0.1 ug reduced purified mouse PD-L1 with histidine tag at C-terminuse
Lane 2 : 0.05 ug reduced purified mousePD-L1 with histidine tag at C-terminus
Dilution : 8 ug/ml

REFERENCE

Application Reference

Zheng F et al. BioMed Research International 2017; 2017 : 1293201 PD-L1 Promotes Self-Renewal and Tumorigenicity of Malignant Melanoma Initiating Cells.
Application : FACS
Reactivity : Mouse
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REVIEW

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Package List Price ($)
$ 399