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PD-L1 antibody [29E.2A3]

Cat. No. GTX01495

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 29E.2A3

Isotype

 IgG2b

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human
Package
100 μg ($129)
See all PD-L1 products
PRODUCT

Summary

The 29E.2A3 antibody reacts with human CD274 which is also known as PD-L1 or B7-H1. CD274 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. PD-L1 is a ligand for CD279 (PD-1) and plays an important role in regulating immune cell responses. CD274 is expressed on activated monocytes, macrophages, dendritic cells, and T cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Inhibition assay Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD274 molecule , B7H , B7H1 , CD274 , PDCD1L1 , PDCD1LG1 , PDL1 , PD-L1

Cellular Localization

Cell membrane,Endomembrane system

Background

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

REFERENCE

Application Reference

Chen JY et al. Br J Cancer 2021; 124 (2) : 484-493 Lysine demethylase 2A expression in cancer-associated fibroblasts promotes breast tumour growth.
Application : ICC/IF
Reactivity : Human

Clone Reference

2019; TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2.
Application : IHC-P
Reactivity : Human
2014; A mechanism of hypoxia-mediated escape from adaptive immunity in cancer cells.
Reactivity : Human
2013; Allogenicity of human cardiac stem/progenitor cells orchestrated by programmed death ligand 1.
Application :
Reactivity : Human
2003; Blockade of programmed death-1 ligands on dendritic cells enhances T cell activation and cytokine production.
Application : IHC-Fr, FACS
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 129