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PD-L1 antibody, C-term

Anti-PD-L1 antibody, C-term used in Western Blot (WB). GTX89590
Anti-PD-L1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89590

Cat No. GTX89590

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Placenta shows membranous staining of cytotrophoblasts.

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89590-PEP).

Product Note

This antibody is expected to recognize reported isoforms a and b (NP_054862.1; NP_001254635.1) only.

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1; NP_001254635.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10723683

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1

Cellular Localization

Cell membrane

Background

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PD-L1 antibody, C-term used in Western Blot (WB). GTX89590

GTX89590 WB Image

WB analysis of human heart lysate using GTX89590 PD-L1 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-PD-L1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89590

GTX89590 IHC-P Image

IHC-P analysis of human placenta using GTX89590 PD-L1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf