PD-L1 antibody [HL1041]

Name: PD-L1 antibody [HL1041]
Brand: GeneTex
SKU: GTX635975
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975

Anti-PD-L1 antibody [HL1041] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635975

Cat. No. GTX635975

Reference 2

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1041
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all PD-L1 products

Datasheet
Reference

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

This antibody is specific for PD-L1 protein , and it does not cross with PD-L2 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909927

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1

Cellular Localization

Cell membrane

Background

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Gene ID: 29126 CD274
UniProt: Q9NZQ7 CD274

Research Area

DATA IMAGES

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Human Placenta.
Green: PD-L1 stained by Dylight594-conjugated PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000.

GTX635975 WB Image

Wild-type (WT) and PDL1 knockout (KO) MDA-MB-231 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

GTX635975 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000 and competitor's antibody diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded A549 (left) and MDA-MB-231 xenograft (right).
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635975 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635975 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX635975 ICC/IF Image

PD-L1 antibody [HL1041] detects PD-L1 protein by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 10 min.
Green: PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma (left) and adjacent macrophages (right).
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lymph node.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human thymus gland.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human ovary.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Abdo M et al. Mol Oncol 2023; 17 (5) : 737-746 Comparative evaluation of PD-L1 expression in cytology imprints, circulating tumour cells and tumour tissue in non-small cell lung cancer patients.

Omenai SA et al. PLoS One 2022; 17 (2) : e0263615 Programme death ligand 1 expressions as a surrogate for determining immunotherapy in cervical carcinoma patients.

Application : IHC-P

Reactivity : Human

Submit a Reference

REVIEW