GeneTex
  • Country / Location Selection

United States (US)

PD-L1 antibody [HL1041]

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975
Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975
Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975

Cat No. GTX635975

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1041

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

This antibody is specific for PD-L1 protein , and it does not cross with PD-L2 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1

Cellular Localization

Cell membrane

Background

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Human Placenta.
Green: PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000.

Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975

GTX635975 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000 and competitor's antibody diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975

GTX635975 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded A549 (left) and MDA-MB-231 xenograft (right).
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD-L1 antibody [HL1041] used in IHC (Paraffin sections) (IHC-P). GTX635975

GTX635975 IHC-P Image

PD-L1 antibody [HL1041] detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
PD-L1 stained by PD-L1 antibody [HL1041] (GTX635975) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody [HL1041] used in Western Blot (WB). GTX635975

GTX635975 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1041] (GTX635975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for PD-L1 antibody [HL1041] (GTX635975). Be the first to share your publications with this product.

REVIEW

PD-L1 antibody [HL1041] Cat No. GTX635975

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 16-Jul-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Placenta tissue
Primary Antibody : 1:1000, 4°C, 16Hr
Package List Price ($)
$ 369
$ 169