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PD-L1 antibody [HL1056]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033
Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033
Anti-PD-L1 antibody [HL1056] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636033
Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033
Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033

Cat. No. GTX636033

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1056

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PD-L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

This antibody was raised against human PD-L1 Intracellular domain, and it does not cross react with human PDL2 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human PD-L1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909937

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1

Cellular Localization

Cell membrane

Background

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033

GTX636033 WB Image

KO/KD

Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033

GTX636033 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody [HL1056] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636033

GTX636033 ICC/IF Image

PD-L1 antibody [HL1056] detects PD-L1 protein by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 10 min.
Green: PD-L1 stained by PD-L1 antibody [HL1056] (GTX636033) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033

GTX636033 WB Image

Comparison Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:1000 and competitor's antibody diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033

GTX636033 WB Image

Orthogonal

Untreated (–) and treated (+) MDA-MB-231 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations