PD-L1 antibody [HL1056]

Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) MDA-MB-231 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:1000 and competitor's antibody diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

PD-L1 antibody [HL1056] detects PD-L1 protein by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 10 min.
Green: PD-L1 stained by PD-L1 antibody [HL1056] (GTX636033) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).