PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 is acceptable but lower pH results in a significant reduction of sensitivity.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48 and BOND RX - Leica. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Alveolar macrophages, tonsil crypt epithelium and the placental cytotrophoblast show strong, predominantly membranous positivity. Follicle centre macrophages should show at least moderate intensity staining.
Negative Control
Appendix (negative staining of all epithelial cells)
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH-conjugated linear peptide corresponding to human PD-L1
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1
Cellular Localization
Cell membrane , Early endosome membrane
Background
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
![Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362](/templates/default/js/lightbox/images/loading.gif "Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362")
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GTX04362 IHC-P Image
IHC-P analysis of human colorectal adenocarcinoma tissue section using GTX04362 PD-L1 antibody [MSVA-711R] HistoMAX™..
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GTX04362 IHC-P Image
IHC-P analysis of human breast cancer tissue section using GTX04362 PD-L1 antibody [MSVA-711R] HistoMAX™..
Breast cancer NST with PD L1 negative tumor cells but intense PD L1 staining in tumor associated inflammatory cells.
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GTX04362 IHC-P Image
IHC-P analysis of human placenta mature tissue section using GTX04362 PD-L1 antibody [MSVA-711R] HistoMAX™..
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REFERENCE
There are currently no references for PD-L1 antibody [MSVA-711R] HistoMAX™ (GTX04362). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PD-L1 antibody [MSVA-711R] HistoMAX™ (GTX04362). Be the first to share your experience with this product.
![Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362](/upload/website/prouct_img/small/GTX04362/GTX04362_20230728_IHC-P_95_23072722_788.webp "Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362")
![Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362](/upload/website/prouct_img/small/GTX04362/GTX04362_20230728_IHC-P_225_23072723_552.webp "Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362")
![Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362](/upload/website/prouct_img/small/GTX04362/GTX04362_20230728_IHC-P_355_23072723_619.webp "Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362")
![Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362](/upload/website/prouct_img/normal/GTX04362/GTX04362_20230728_IHC-P_95_23072722_788.webp "Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362")
![Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362](/upload/website/prouct_img/normal/GTX04362/GTX04362_20230728_IHC-P_225_23072723_552.webp "Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362")
![Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362](/upload/website/prouct_img/normal/GTX04362/GTX04362_20230728_IHC-P_355_23072723_619.webp "Anti-PD-L1 antibody [MSVA-711R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04362")