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PD-L1 antibody

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763

Cat No. GTX104763

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Observed MW

50 kDa. The observed M.W. is based on the following publication. PMID: 23674495

Positive Control

A431 , MDA-MB-231 , MDA-MB-231 (24μg/ml Tunicamycin treatment for 16 hr) , MDA-MB-231 , A549 (100 ng/mL IFN-gamma treatment for 48 hr) , A431 (100 ng/mL IFN-gamma treatment for 48 hr)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd274 Molecule,B7-H,B7H1,Pd-L1,Pdcd1L1,Pdcd1Lg1,Pdl1,Cd274

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Endomembrane system; Single-pass type I membrane protein

Background

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Database

Research Area

DATA IMAGES
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Untreated (–) and treated (+) A549 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:600 and competitor's antibody (CST#13684) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:1200 and competitor's antibody (CST#13684) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:600 and with DDDDK tag antibody (GTX115043) diluted at 1:3000 to detect DDDDK-tagged PD-L2. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000.

Anti-PD-L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104763

GTX104763 ICC/IF Image

PD-L1 antibody detects PD-L1 protein at cell membrane in MDA-MB-231 cells by immunofluorescent analysis.
Sample: MDA-MB-231 (left) and HeLa (right) cells.
Green: PD-L1 protein stained by PD-L1 antibody (GTX104763) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763

GTX104763 IHC-P Image

PD-L1 antibody detects PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
PD-L1 antibody (GTX104763) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763

GTX104763 IHC-P Image

PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:2000 and competitor's antibody (CST#13684) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763

GTX104763 IHC-P Image

PD-L1 antibody detects PD-L1 protein at cell membrane in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50. Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE
REVIEW

PD-L1 antibody Cat No. GTX104763

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 22-May-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SK-LMS-1 cells
Amount used : 30 μg
Blocking : 3%BSA, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf