GeneTex
United States (US)

PD-L1 antibody

KO/KD Comparison Orthogonal
GTX104763_42775_20170407_WB_IFN-gamma_1_19011506_625.jpg
GTX104763_42775_20170407_WB_IFN-gamma_2_19011506_447.jpg
GTX104763_42775_20170428_WB_Specificity_19011506_154.jpg
GTX104763_42775_20170216_WB_Tunicamycin_19011506_105.jpg
GTX104763_42865_20170720_IFA_19011506_391.jpg
GTX104763_42775_20170407_WB_shRNA_watermark_19011506_307.jpg
GTX104763_42795_20170419_IHC-P_19011506_331.jpg
GTX104763_42795_20170419_IHC-P_competitor_2_19011506_660.jpg
GTX104763_42795_20170419_IHC-P_competitor_19011506_724.jpg
GTX104763_43334_20180831_WB_19011506_883.jpg
GTX104763_42795_20170525_WB_competitor_watermark_19011506_551.jpg

Cat No. GTX104763

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Observed MW

50 kDa. The observed M.W. is based on the following publication. PMID: 23674495

Positive Control

A431 , MDA-MB-231 , MDA-MB-231 (24μg/ml Tunicamycin treatment for 16 hr) , MDA-MB-231 , A549 (100 ng/mL IFN-gamma treatment for 48 hr) , A431 (100 ng/mL IFN-gamma treatment for 48 hr)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B7-H antibody, B7H1 antibody, MGC142294 antibody, MGC142296 antibody, PD-L1 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL1 antibody, CD274 antibody, B7 homolog 1 antibody, programmed death ligand 1 antibody, programmed cell death 1 ligand 1 antibody, PDCD1 ligand 1 antibody, CD274 antigen antibody, CD274 molecule antibody

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Endomembrane system; Single-pass type I membrane protein

Background

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Database

Research Area

DATA IMAGES
GTX104763_42775_20170407_WB_IFN-gamma_1_19011506_625.jpg

GTX104763 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) A549 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:600 and competitor's antibody (CST#13684) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX104763_42775_20170407_WB_IFN-gamma_2_19011506_447.jpg

GTX104763 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:1200 and competitor's antibody (CST#13684) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX104763_42775_20170428_WB_Specificity_19011506_154.jpg

GTX104763 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:600 and with DDDDK tag antibody (GTX115043) diluted at 1:3000 to detect DDDDK-tagged PD-L2. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104763_42775_20170216_WB_Tunicamycin_19011506_105.jpg

GTX104763 WB Image

Orthogonal

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000.

GTX104763_42865_20170720_IFA_19011506_391.jpg

GTX104763 ICC/IF Image

PD-L1 antibody detects PD-L1 protein at cell membrane in MDA-MB-231 cells by immunofluorescent analysis.
Sample: MDA-MB-231 (left) and HeLa (right) cells.
Green: PD-L1 protein stained by PD-L1 antibody (GTX104763) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX104763_42775_20170407_WB_shRNA_watermark_19011506_307.jpg

GTX104763 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104763_42795_20170419_IHC-P_19011506_331.jpg

GTX104763 IHC-P Image

PD-L1 antibody detects PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
PD-L1 antibody (GTX104763) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104763_42795_20170419_IHC-P_competitor_2_19011506_660.jpg

GTX104763 IHC-P Image

Comparison

PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX104763_42795_20170419_IHC-P_competitor_19011506_724.jpg

GTX104763 IHC-P Image

Comparison

PD-L1 antibody detects PD-L1 protein at cell membrane in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50. Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX104763_43334_20180831_WB_19011506_883.jpg

GTX104763 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX104763_42795_20170525_WB_competitor_watermark_19011506_551.jpg

GTX104763 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:2000 and competitor's antibody (CST#13684) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE
REVIEW

PD-L1 antibody Cat No. GTX104763

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 22-May-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SK-LMS-1 cells
Amount used : 30 μg
Blocking : 3%BSA, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf