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PD-L1 antibody

KO/KD Orthogonal Overexpression
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in Flow cytometry (FACS). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

Cat. No. GTX31308

Reference 5
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, ELISA, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($419)
See all PD-L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
FACS 0.5 μg/ml
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Raji Cell Lysate(GTX27908) , Human Heart Tissue Lysate(GTX27916) , Human Heart Tissue Slide

Product Note

PD-L1 antibody has no cross-reactivity to PD-L2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PD-L1 antibody was raised against a 17 amino acid synthetic peptide from near the center of human PD-L1.The immunogen is located within amino acids 60 - 110 of PD-L1(Accession number: NP_054862).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887613

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody.
Red : Primary antibody
Dilution : 1:250

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 5 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

WB analysis of various sample lysates using GTX31308 PD-L1 antibody.
Loading : 15μg of lysates per lane
Dilution : 2 μg/ml

Anti-PD-L1 antibody used in Flow cytometry (FACS). GTX31308

GTX31308 FACS Image

FACS analysis of A-20 cells using GTX31308 PD-L1 antibody.
Working concentration : 0.5 μg/ml
Green: Isotype control
Red : PD-L1 antibody

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody.
Working concentration : 20 μg/ml

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX31308 PD-L1 antibody.
Working concentration : 5 μg/ml

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody.
Working concentration : 2.5 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

WB analysis of Raji cell lysate using GTX31308 PD-L1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

Overexpression

WB analysis of PD-L1 overexpression in 293 cells using GTX31308 PD-L1 antibody.
Lane 1 : non-transfected 293 cells
Lane 2 : PD-L1 overexpressed 293 cells
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

KO/KD

WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2) using GTX31308 PD-L1 antibody.
Loading : 10μg of lysates per lane
Dilution : 2 μg/ml

REFERENCE

Application Reference

Wu M et al. Nat Commun 2021; 12 (1) : 3500 WSX1 act as a tumor suppressor in hepatocellular carcinoma by downregulating neoplastic PD-L1 expression.
Qin G et al. Nat Commun 2020; 11 (1) : 1669 NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer.
Liu BL et al. JCI Insight 2020; 5 (20) Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia.
Application : WB
Reactivity : Mouse
Chang HL et al. Int J Med Sci 2020; 17 (7) : 939-945 The Extracts of Astragalus membranaceus Overcome Tumor Immune Tolerance by Inhibition of Tumor Programmed Cell Death Protein ligand-1 Expression.
Application : WB
Reactivity : Mouse
Wang LA et al. Tumour Biol 2015; The prediction of survival of patients with gastric cancer with PD-L1 expression using contrast-enhanced ultrasonography.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

PD-L1 antibody Cat. No. GTX31308

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020.PMID: 32245950
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020.PMID: 32245950
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Date : Anonymous submitted on 07-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : GL261 cells
Amount used : 30 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Secondary Antibody : GTX300119, 1:1000, 25°C, 1Hr
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419