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PD-L1 antibody

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in Flow cytometry (FACS). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

Cat. No. GTX31308

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS, ELISA, IHC

Reactivity

Human, Mouse, Rat
Package
100 μg ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
FACS 0.5 μg/ml
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Raji Cell Lysate(GTX27908) , Human Heart Tissue Lysate(GTX27916) , Human Heart Tissue Slide

Product Note

PD-L1 antibody has no cross-reactivity to PD-L2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PD-L1 antibody was raised against a 17 amino acid synthetic peptide from near the center of human PD-L1.The immunogen is located within amino acids 60 - 110 of PD-L1(Accession number: NP_054862).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887613

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody.
Working concentration : 2.5 μg/ml

Anti-PD-L1 antibody used in Flow cytometry (FACS). GTX31308

GTX31308 FACS Image

FACS analysis of A-20 cells using GTX31308 PD-L1 antibody.
Working concentration : 0.5 μg/ml
Green: Isotype control
Red : PD-L1 antibody

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody.
Red : Primary antibody
Dilution : 1:250

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX31308 PD-L1 antibody.
Working concentration : 5 μg/ml

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 5 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

WB analysis of Raji cell lysate using GTX31308 PD-L1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2) using GTX31308 PD-L1 antibody.
Loading : 10μg of lysates per lane
Dilution : 2 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

WB analysis of various sample lysates using GTX31308 PD-L1 antibody.
Loading : 15μg of lysates per lane
Dilution : 2 μg/ml

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody.
Working concentration : 20 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

WB analysis of PD-L1 overexpression in 293 cells using GTX31308 PD-L1 antibody.
Lane 1 : non-transfected 293 cells
Lane 2 : PD-L1 overexpressed 293 cells
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

REFERENCE

REVIEW

PD-L1 antibody Cat. No. GTX31308

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020.PMID: 32245950
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2020.PMID: 32245950
Date : Anonymous submitted on 07-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : GL261 cells
Amount used : 30 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Secondary Antibody : GTX300119, 1:1000, 25°C, 1Hr
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419