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PD-L1 antibody

KO/KD Orthogonal Overexpression
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in Flow cytometry (FACS). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

Cat. No. GTX31308

Reference 7
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, ELISA, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($419)
See all PD-L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
FACS 0.5 μg/ml
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Raji Cell Lysate(GTX27908) , Human Heart Tissue Lysate(GTX27916) , Human Heart Tissue Slide

Product Note

PD-L1 antibody has no cross-reactivity to PD-L2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PD-L1 antibody was raised against a 17 amino acid synthetic peptide from near the center of human PD-L1.The immunogen is located within amino acids 60 - 110 of PD-L1(Accession number: NP_054862).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887613

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody.
Working concentration : 2.5 μg/ml

Anti-PD-L1 antibody used in Flow cytometry (FACS). GTX31308

GTX31308 FACS Image

FACS analysis of A-20 cells using GTX31308 PD-L1 antibody.
Working concentration : 0.5 μg/ml
Green: Isotype control
Red : PD-L1 antibody

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody.
Red : Primary antibody
Dilution : 1:250

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX31308 PD-L1 antibody.
Working concentration : 5 μg/ml

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 5 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

WB analysis of Raji cell lysate using GTX31308 PD-L1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

KO/KD

WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2) using GTX31308 PD-L1 antibody.
Loading : 10μg of lysates per lane
Dilution : 2 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

WB analysis of various sample lysates using GTX31308 PD-L1 antibody.
Loading : 15μg of lysates per lane
Dilution : 2 μg/ml

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31308

GTX31308 IHC-P Image

IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody.
Working concentration : 20 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

Overexpression

WB analysis of PD-L1 overexpression in 293 cells using GTX31308 PD-L1 antibody.
Lane 1 : non-transfected 293 cells
Lane 2 : PD-L1 overexpressed 293 cells
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2020.PMID: 32245950

Anti-PD-L1 antibody used in Western Blot (WB). GTX31308

GTX31308 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2020.PMID: 32245950

REFERENCE

Application Reference

G Qin et al. Mol Med 2024; 30 (1) : 13 Targeting the NAT10/NPM1 axis abrogates PD-L1 expression and improves the response to immune checkpoint blockade therapy
Y Liu et al. Cell Rep 2023; 42 (11) : 113343 PD-L1-mediated immune evasion in triple-negative breast cancer is linked to the loss of ZNF652
Reactivity : Mouse
Wu M et al. Nat Commun 2021; 12 (1) : 3500 WSX1 act as a tumor suppressor in hepatocellular carcinoma by downregulating neoplastic PD-L1 expression.
Qin G et al. Nat Commun 2020; 11 (1) : 1669 NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer.
Liu BL et al. JCI Insight 2020; 5 (20) Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia.
Application : WB
Reactivity : Mouse
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REVIEW

PD-L1 antibody Cat. No. GTX31308

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-PD-L1 antibody used in Western Blot (WB). GTX31308
Date : Anonymous submitted on 07-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : GL261 cells
Amount used : 30 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Secondary Antibody : GTX300119, 1:1000, 25°C, 1Hr
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419