GeneTex
United States (US)

PD-L2 antibody

GTX85449_2243_IHC-P_20180221_18121411_353.jpg
GTX85449_3701_WB_20180221_18121411_655.jpg
GTX85449_2244_IHC-P_20180221_18121411_997.jpg
GTX85449_2242_IHC-P_20180221_18121411_473.jpg

Cat No. GTX85449

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Raji Cell Lysate(GTX27908) , Mouse Brain Tissue Lysate(GTX27934)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PD-L2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-L2.The immunogen is located within amino acids 140 - 190 of PD-L2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PDL2, PDL-2, Programmed cell death 1 ligand2, B7DC, programmed death ligand1, programmed death ligand 1, PD-L2, Programmed cell death1 ligand2, Programmed cell death 1 ligand-2, PDL 2, PD L2

Background

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PDL-2 can act to stimulate an immunogenic response through and alternative receptor from PD-1. At least three isoforms of PDL-2 are known to exist; this antibody is specific to the largest isoform. PDL-2 antibody has no cross-reactivity to PDL-2.

Database

Research Area

DATA IMAGES
GTX85449_2243_IHC-P_20180221_18121411_353.jpg

GTX85449 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85449 PD-L2 antibody.
Working concentration : 2.5 μg/ml

GTX85449_3701_WB_20180221_18121411_655.jpg

GTX85449 WB Image

WB analysis of Raji cell lysate using GTX85449 PD-L2 antibody.
Working concentration : 0.5 and 1 μg/ml

GTX85449_2244_IHC-P_20180221_18121411_997.jpg

GTX85449 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85449 PD-L2 antibody.
Working concentration : 20 μg/ml

GTX85449_2242_IHC-P_20180221_18121411_473.jpg

GTX85449 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85449 PD-L2 antibody.
Working concentration : 2.5 μg/ml