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PD1 antibody [4F12]

Anti-PD1 antibody [4F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50053
Anti-PD1 antibody [4F12] used in Flow cytometry (FACS). GTX50053
Anti-PD1 antibody [4F12] used in Western Blot (WB). GTX50053

Cat. No. GTX50053

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F12

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all PD1 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:500 ~ 1,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PDCD1 (21-167aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

programmed cell death 1 , CD279 , PD-1 , PD1 , SLEB2 , hPD-1 , hPD-l , hSLE1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PD1 antibody [4F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50053

GTX50053 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX50053 PD1 antibody [4F12].
Green : Primary antibody
Blue : cell nucleus
Dilution : 1:100

Anti-PD1 antibody [4F12] used in Flow cytometry (FACS). GTX50053

GTX50053 FACS Image

FACS analysis of HeLa cells using GTX50053 PD1 antibody [4F12].
Red : Primary antibody
Black : Isotype control
Dilution : 2-5μg for 1x10⁶ cells

Anti-PD1 antibody [4F12] used in Western Blot (WB). GTX50053

GTX50053 WB Image

WB analysis of various cell lysate using GTX50053 PD1 antibody [4F12].
Lane 1 : 293T cell lysate
Lane 2 : Raji cell lysate
Dilution : 1:3000
Loading : 40μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399