PD1 antibody [HL4386]

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD1 antibody [HL4386] (GTX643643) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Boiled and unboiled RPMI-8226 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD1 antibody [HL4386] (GTX643643) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD1 antibody [HL4386] (GTX643643) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PD1 antibody [HL4386] detects PD1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
PD1 stained by PD1 antibody [HL4386] (GTX643643) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min