GeneTex
United States (US)

PD1 antibody

GTX128435_41241_20160309_IHC-P_18121410_427.jpg
GTX128435_41241_20151015_WB_18121410_223.jpg
GTX128435_41241_20180306_IHC-P_M_1_18121410_164.jpg
GTX128435_41241_20180306_IHC-P_M_18121410_978.jpg
GTX128435_41241_WB_18121410_904.jpg
GTX128435_41241_20180202_WB_B_18121410_395.jpg

Cat No. GTX128435

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

NCI-H929 , DDDDK-tagged PD1-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD279 antibody, PD-1 antibody, PD1 antibody, SLEB2 antibody, hPD-1 antibody, hPD-l antibody, PDCD1 antibody, programmed cell death protein 1 antibody, protein PD-1 antibody, programmed cell death 1 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX128435_41241_20160309_IHC-P_18121410_427.jpg

GTX128435 IHC-P Image

PD1 antibody detects PD1 protein at cell membrane of inflammatory cells in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PD1 antibody (GTX128435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128435_41241_20151015_WB_18121410_223.jpg

GTX128435 WB Image

PD-1 antibody detects PD-1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PD-1 antibody (GTX128435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128435_41241_20180306_IHC-P_M_1_18121410_164.jpg

GTX128435 IHC-P Image

PD1 antibody detects PD1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
PD1 stained by PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128435_41241_20180306_IHC-P_M_18121410_978.jpg

GTX128435 IHC-P Image

PD1 antibody detects PD1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
PD1 stained by PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128435_41241_WB_18121410_904.jpg

GTX128435 WB Image

PD1 antibody detects PDCD1 protein by western blot analysis.
A. 30 µg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
PD1 antibody (GTX128435) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128435_41241_20180202_WB_B_18121410_395.jpg

GTX128435 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD1 antibody (GTX128435) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf