APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NCI-H929 , HL-60 , DDDDK-tagged PD1-transfected 293T
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PD1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885778
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
programmed cell death 1 , CD279 , PD-1 , PD1 , SLEB2 , hPD-1 , hPD-l , hSLE1
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX128435 IHC-P Image
PD1 antibody detects PD1 protein at cell membrane of inflammatory cells in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX128435 WB Image
PD-1 antibody detects PD-1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PD-1 antibody (GTX128435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128435 IHC-P Image
PD1 antibody detects PD1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse lymph node.PD1 stained by PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX128435 IHC-P Image
PD1 antibody detects PD1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.PD1 stained by PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX128435 WB Image
PD1 antibody detects PDCD1 protein by western blot analysis.
A. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
PD1 antibody (GTX128435) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128435 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD1 antibody (GTX128435) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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