GeneTex
United States (US)

PD1 antibody

Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31309
Anti-PD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31309
Anti-PD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31309
Anti-PD1 antibody used in Western Blot (WB). GTX31309

Cat No. GTX31309

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 10 μg/ml
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , THP-1 Cell Lysate(GTX27913)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PD-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-1.The immunogen is located within amino acids 120 - 170 of PD-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Programmed Cell Death 1,Cd279,Pd-1,Pd1,Sleb2,Hpd-1,Hpd-L,Hsle1,Pdcd1

Background

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31309

GTX31309 IHC-P Image

IHC-P analysis of human brain tissue using GTX31309 PD1 antibody.
Working concentration : 5 μg/ml

Anti-PD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31309

GTX31309 ICC/IF Image

ICC/IF analysis of PD-1-transfected HEK293 cells using GTX31309 PD1 antibody.
Working concentration : 20 μg/ml

Anti-PD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31309

GTX31309 ICC/IF Image

ICC/IF analysis of PD-1-transfected HEK293 cells using GTX31309 PD1 antibody.
Working concentration : 10 μg/ml

Anti-PD1 antibody used in Western Blot (WB). GTX31309

GTX31309 WB Image

WB analysis of THP-1 cell lysate in the (A) absence and (B) presence of blocking peptide using GTX31309 PD1 antibody.
Working concentration : 1 μg/ml