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PD1 antibody

Anti-PD1 antibody used in Western Blot (WB). GTX31756
Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31756
Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31756
Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31756
Anti-PD1 antibody used in Western Blot (WB). GTX31756

Cat No. GTX31756

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , THP-1 Cell Lysate(GTX27913)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PD-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PD-1.The immunogen is located within amino acids 210 - 260 of PD-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

programmed cell death 1 , CD279 , PD-1 , PD1 , SLEB2 , hPD-1 , hPD-l , hSLE1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PD1 antibody used in Western Blot (WB). GTX31756

GTX31756 WB Image

WB analysis of THP-1 cell lysate in the (A) absence and (B) presence of blocking peptide using GTX31756 PD1 antibody.
Working concentration : 1 μg/ml

Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31756

GTX31756 IHC-P Image

IHC-P analysis of human brain tissue using GTX31756 PD1 antibody.
Working concentration : 20 μg/ml

Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31756

GTX31756 IHC-P Image

IHC-P analysis of human brain tissue using GTX31756 PD1 antibody.
Working concentration : 2.5 μg/ml

Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31756

GTX31756 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX31756 PD1 antibody.
Working concentration : 5 μg/ml

Anti-PD1 antibody used in Western Blot (WB). GTX31756

GTX31756 WB Image

WB analysis of rat thymus cell lysate using GTX31756 PD1 antibody.
Dilution : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339