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PDCD4 antibody, N-term

Anti-PDCD4 antibody, N-term used in Western Blot (WB). GTX52831
Anti-PDCD4 antibody, N-term used in Western Blot (WB). GTX52831

Cat. No. GTX52831

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μg ($399)
See all PDCD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HepG2 , Jurkat

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human PDCD4

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_626020

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

programmed cell death 4 , H731

Cellular Localization

Nucleus,Cytoplasm

Background

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-PDCD4 antibody, N-term used in Western Blot (WB). GTX52831

GTX52831 WB Image

WB analysis of Jurkat cells using GTX52831 PDCD4 antibody at 0.2-1μg/ml.

Anti-PDCD4 antibody, N-term used in Western Blot (WB). GTX52831

GTX52831 WB Image

WB analysis of HepG2 cells using GTX52831 PDCD4 antibody at 2μg/ml.
Lane A : Primary antibody
Lane B : Primary antibody + blocking peptide

REFERENCE

Application Reference

Manavalan TT et al. Cancer Lett 2011; 313 (1) : 26-43 differential expression of microrna expression in *-sensitive mcf-7 versus *-resistant ly2 human breast cancer cells.
Application : WB
Reactivity : Human
Klinge CM et al. Mol Cell Endocrinol 2010; 323 (2) : 268-76 estrogen receptor alpha 46 is reduced in * resistant breast cancer cells and re-expression inhibits cell proliferation and estrogen receptor alpha 66-regulated target gene transcription.
Application : WB
Reactivity : Human
Wickramasinghe NS et al. Nucleic Acids Res 2009; 37 (8) : 2584-95 * downregulates mir-21 expression and increases mir-21 target gene expression in mcf-7 breast cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399