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PDCD4 antibody [k4C1]

Anti-PDCD4 antibody [k4C1] used in Western Blot (WB). GTX50048

Cat. No. GTX50048

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 k4C1

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all PDCD4 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000~ 3,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PDCD4 (1-469aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

programmed cell death 4 , H731

Cellular Localization

Nucleus , Cytoplasm

Background

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-PDCD4 antibody [k4C1] used in Western Blot (WB). GTX50048

GTX50048 WB Image

Cell lysates of HepG2(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399