GeneTex
United States (US)

PDCD4 antibody

GTX104901_40723_20151022_WB_M_heart_19011506_793.jpg
GTX104901_40723_WB_19011506_512.jpg
GTX104901_40723_IFA_19011506_192.jpg

Cat No. GTX104901

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

A431 , HeLa , Mouse heart

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDCD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H731 antibody, MGC33046 antibody, MGC33047 antibody, PDCD4 antibody, nuclear antigen H731 antibody, protein 197/15a antibody, neoplastic transformation inhibitor protein antibody, programmed cell death protein 4 antibody, programmed cell death 4 (neoplastic transformation inhibitor) antibody

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104901_40723_20151022_WB_M_heart_19011506_793.jpg

GTX104901 WB Image

PDCD4 antibody detects PDCD4 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 antibody (GTX104901) diluted at 1:1000.

GTX104901_40723_WB_19011506_512.jpg

GTX104901 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
10% SDS PAGE
GTX104901 diluted at 1:1000

GTX104901_40723_IFA_19011506_192.jpg

GTX104901 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PDCD4(GTX104901) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf