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PDCD4 (phospho Ser457) antibody [2E10.C12]

Cat No. GTX48644

Host

Mouse

Clonality

Monoclonal

Clone Name

2E10.C12

Isotype

IgG1

Application

WB, ELISA, IHC

Reactivity

Human, Mouse, Rat, Xenopus laevis
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:3000
ELISA 1:400000
IHC 1.25 - 2.5 μg/mL
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Anti-Pdcd4 phospho S457 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

programmed cell death 4 , H731

Cellular Localization

Nucleus,Cytoplasm

Background

Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

Database

Research Area

REFERENCE

There are currently no references for PDCD4 (phospho Ser457) antibody [2E10.C12] (GTX48644). Be the first to share your publications with this product.

REVIEW

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SDS
Sodium Azide.pdf