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PDCD4 (phospho Ser457) antibody

Orthogonal
Anti-PDCD4 (phospho Ser457) antibody used in IHC (Paraffin sections) (IHC-P). GTX132798
Anti-PDCD4 (phospho Ser457) antibody used in Western Blot (WB). GTX132798
Anti-PDCD4 (phospho Ser457) antibody used in IHC (Paraffin sections) (IHC-P). GTX132798
Anti-PDCD4 (phospho Ser457) antibody used in Western Blot (WB). GTX132798

Cat. No. GTX132798

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PDCD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

NIH-3T3(starvation 16 hours and 5 ng/ml PDGF treatment for 24 hr) , PC-12 , Rat2

Predict Reactivity

Human, Drosophila, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser457 of human PDCD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886748

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

programmed cell death 4 , H731

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PDCD4 (phospho Ser457) antibody used in IHC (Paraffin sections) (IHC-P). GTX132798

GTX132798 IHC-P Image

PDCD4 (phospho Ser457) antibody detects PDCD4 (phospho Ser457) protein at nucleus in mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
PDCD4 (phospho Ser457) antibody (GTX132798) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDCD4 (phospho Ser457) antibody used in Western Blot (WB). GTX132798

GTX132798 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody (GTX132798) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDCD4 (phospho Ser457) antibody used in IHC (Paraffin sections) (IHC-P). GTX132798

GTX132798 IHC-P Image

PDCD4 (phospho Ser457) antibody detects PDCD4 (phospho Ser457) protein at nucleus in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
PDCD4 (phospho Ser457) antibody (GTX132798) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDCD4 (phospho Ser457) antibody used in Western Blot (WB). GTX132798

GTX132798 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody (GTX132798) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169