GeneTex
United States (US)

PDCD6 antibody

GTX102195_40562_IFA_19011506_616.jpg
GTX102195_40562_WB_19011506_167.jpg
GTX102195_40562_20141128_IHC_19011506_445.jpg

Cat No. GTX102195

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

HeLa , HeLa nucleus

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDCD6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALG-2 antibody, FLJ46208 antibody, MGC111017 antibody, MGC119050 antibody, MGC9123 antibody, PEF1B antibody, PDCD6 antibody, apoptosis-linked gene-2 antibody, apoptosis-linked gene 2 protein antibody, programmed cell death protein 6 antibody, probable calcium-binding protein ALG-2 antibody, programmed cell death 6 antibody

Cellular Localization

Nucleus membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus

Background

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX102195_40562_IFA_19011506_616.jpg

GTX102195 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PDCD6(GTX102195) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX102195_40562_WB_19011506_167.jpg

GTX102195 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX102195 diluted at 1:500

GTX102195_40562_20141128_IHC_19011506_445.jpg

GTX102195 IHC-P Image

PDCD6 antibody detects PDCD6 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
PDCD6 antibody (GTX102195) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf