APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:100 |
1:50 - 1:200 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human PDCD6 (NP_037364.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
programmed cell death 6 , ALG-2 , ALG2 , PEF1B
Cellular Localization
Endoplasmic reticulum membrane,Cytoplasm,Nucleus,Endosome
Background
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5. [provided by RefSeq, May 2012]
Database
Research Area
DATA IMAGES
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GTX33394 WB Image
WB analysis of various sample lysates using GTX33394 PDCD6 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX33394 IP Image
IP analysis of HepG2 cell lysate using GTX33394 PDCD6 antibody. Antibody amount : 3μg / 200μg lysate Dilution : 1:1000
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GTX33394 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX33394 PDCD6 antibody. Blue : DAPI
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GTX33394 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX33394 PDCD6 antibody. Dilution : 1:200
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REFERENCE
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REVIEW
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