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PDE10A antibody [C2C3], C-term

Anti-PDE10A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886
Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886

Cat No. GTX118886

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

HCT116 , mouse brain , rat brain , Myc-DDK-tagged PDE10A-transfected 293T

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PDE10A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphodiesterase 10A , ADSD2 , HSPDE10A , IOLOD , LINC00473 , PDE10A19

Cellular Localization

Cytoplasm

Background

Various cellular responses are regulated by the second messengers cAMP and cGMP. Phosphodiesterases, such as PDE10A, eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate (Fujishige et al., 2000 [PubMed 10998054]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PDE10A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX118886

GTX118886 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PDE10A(GTX118886) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PDE10A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118886

GTX118886 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PDE10A(GTX118886) antibody at 1:200 dilution.

Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886

GTX118886 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886

GTX118886 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000 and competitor's antibody (ab177933) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886

GTX118886 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE10A antibody [C2C3], C-term used in Western Blot (WB). GTX118886

GTX118886 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169