For IHC: Use at an assay dependent dilution. For IP: Use at a dilution of 1:250. For WB: Use at a dilution of 1:500. Detects a band of approximately 90-93 kDa in rat brain and rat pancreas samples. Optimal dilutions/concentrations should be determined by the researcher.
For western blot - Rat brain and pancreas
This antibody has no cross-reactivity against any other PDE family members tested so far.
‘Stabilization buffer' with 0.02% thimerosal or merthiolate.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide (Human) against a common sequence near the 250-325 amino acids downstream from the N terminal region of PDE10A.
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Located mostly to soluble cellular fractions.
One of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is PDE10. Only one member of the cAMP and cGMP-specific PDE10 family has been cloned and characterized. The PDE10A gene is expressed in at least two variant forms (PDE10A1, PDE10A2) generated by RNA splicing and/or the use of alternate initiation sites. PDE10A is widely expressed in the putamen and caudate nucleus in the brain, thyroid and testis. The expression of PDE10A is also abundant in Heart.
GTX14622 WB Image
Western using GTX14622 and various rat brain regions. GTX14622 antibody 1:500 diluted.
1. Frontal Cortex
2. Parietal Cortex
7. Olfactory Bulb
8. Brain Stem
10. Spinal Cord
11. Whole Brain