GeneTex
United States (US)

PDE1A antibody, C-term

GTX88149_1034_IHC-P_18122102_767.jpg
GTX88149_2468_WB_18122102_921.jpg

Cat No. GTX88149

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88149-PEP).

Specificity/Sensitivity

This antibody is expected to recognize isoform 1 (NP_005010.2) only.

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DLHKNSEDLVNAE, from the C Terminus of the protein sequence according to NP_005010.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphodiesterase 1A,Cam-Pde 1A,Cam-Pde-1A,Hcam-1,Hcam1,Hspde1A,Pde1A

Background

Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]

Database

Research Area

DATA IMAGES
GTX88149_1034_IHC-P_18122102_767.jpg

GTX88149 IHC-P Image

IHC-P analysis of human kidney using GTX88149 PDE1A antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88149_2468_WB_18122102_921.jpg

GTX88149 WB Image

WB analysis of human cerebellum lysate using GTX88149 PDE1A antibody, C-term.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329