Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE1A antibody (GTX111687) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using PDE1A(GTX111687) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
GTX111687 diluted at 1:1000