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PDE4A antibody [7B11]

Overexpression
Anti-PDE4A antibody [7B11] used in Flow cytometry (FACS). GTX83922
Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922
Anti-PDE4A antibody [7B11] used in Western Blot (WB). GTX83922
Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922
Anti-PDE4A antibody [7B11] used in Western Blot (WB). GTX83922
Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922
Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922
Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922
Anti-PDE4A antibody [7B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83922
Anti-PDE4A antibody [7B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83922
Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922
Anti-PDE4A antibody [7B11] used in Flow cytometry (FACS). GTX83922

Cat. No. GTX83922

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7B11

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all PDE4A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:4000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 4A , DPDE2 , PDE4 , PDE46

Cellular Localization

Membrane

Background

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Database

Research Area

DATA IMAGES

Anti-PDE4A antibody [7B11] used in Flow cytometry (FACS). GTX83922

GTX83922 FACS Image

FACS analysis of HEK293T cells transfected with either PDE4A plasmid(Red) or empty vector control plasmid(Blue) using GTX83922 PDE4A antibody [7B11].

Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922

GTX83922 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PDE4A antibody [7B11] used in Western Blot (WB). GTX83922

GTX83922 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PDE4A plasmid (Right) or empty vector (Left) for 48 hrs using GTX83922 PDE4A antibody [7B11].
Loading : 5 ug per lane

Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922

GTX83922 IHC-P Image

IHC-P analysis of human colon tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PDE4A antibody [7B11] used in Western Blot (WB). GTX83922

GTX83922 WB Image

WB analysis of various cell lines using GTX83922 PDE4A antibody [7B11].
Loading : 10 ug per lane
Dilution : 1:200

Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922

GTX83922 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922

GTX83922 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922

GTX83922 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PDE4A antibody [7B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83922

GTX83922 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PDE4A plasmid using GTX83922 PDE4A antibody [7B11].

Anti-PDE4A antibody [7B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83922

GTX83922 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83922 PDE4A antibody [7B11].

Anti-PDE4A antibody [7B11] used in IHC (Paraffin sections) (IHC-P). GTX83922

GTX83922 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PDE4A antibody [7B11] used in Flow cytometry (FACS). GTX83922

GTX83922 FACS Image

FACS analysis of Jurkat cells using GTX83922 PDE4A antibody [7B11].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519