GeneTex
United States (US)

PDE4A antibody

PDE4A-antibody_WB_GTX14607-1_18121411_831.jpg

Cat No. GTX14607

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat, Rabbit
APPLICATION

Application Note

ICC: Use at a dilution of 1/250. IF: Use at a dilution of 1/250. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/1000. Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

98 kDa. ( Note )

Specificity/Sensitivity

This antibody labels all known PDE4A variants including PDE4A1 (66 kDa), A5 (109 kDa), A8 (106 kDa), Ax (102 kDa) and a 76 kDa testis specific A variant.
PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the C-terminal region of PDE4A (common to all PDE4A proteins).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphodiesterase 4A,Dpde2,Pde4,Pde46,Pde4A

Cellular Localization

Cell Membrane

Background

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling.

Database

Research Area

DATA IMAGES
PDE4A-antibody_WB_GTX14607-1_18121411_831.jpg

GTX14607 WB Image

Western blot of PDE4A using GTX14607