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PDE4A (phospho Ser140) antibody

Anti-PDE4A (phospho Ser140) antibody used in Western Blot (WB). GTX60930
Anti-PDE4A (phospho Ser140) antibody used in Western Blot (WB). GTX60930
Anti-PDE4A (phospho Ser140) antibody used in Western Blot (WB). GTX60930

Cat. No. GTX60930

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Human, Mouse, Rat, Rabbit
Package
50 μg ($399)
See all PDE4A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
IP 1:150
ELISA 1:10000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Product Note

This antibody detects all known long-form variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phosphorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with other members of the PDE family including PDE4B or PDE4D.

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine, 0.5% BSA, 30% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine residue.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 4A , DPDE2 , PDE4 , PDE46

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-PDE4A (phospho Ser140) antibody used in Western Blot (WB). GTX60930

GTX60930 WB Image

WB analysis of PDE4A5 recombinant protein with or without PKA-dependent phosphorylation using GTX60930 PDE4A (phospho Ser140) antibody.
Dilution : 1:500

Anti-PDE4A (phospho Ser140) antibody used in Western Blot (WB). GTX60930

GTX60930 WB Image

WB analysis of phosphorylated and nonphosphorylated PDE4D protein using GTX60930 PDE4A (phospho Ser140) antibody.
Dilution : 1:500

Anti-PDE4A (phospho Ser140) antibody used in Western Blot (WB). GTX60930

GTX60930 WB Image

WB analysis of increased phosphorylation of PDE4AG in short-term FSK stimulation using GTX60930 PDE4A (phospho Ser140) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399