GeneTex
United States (US)

PDE4A4 antibody

Cat No. GTX14609

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rabbit
APPLICATION

Application Note

ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/500. Detects a band of approximately 125 kDa. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

U937 cells, human SHSY-5Y and Jurkat cell extracts

Specificity/Sensitivity

Does not cross react with PDE4A1, A8 or A5 proteins and PDE4B, PDE4C and PDE4D or with other PDE family members.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Thimerosal / Merthiolate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

N-terminus PDE4A4 synthetic cyclic peptide.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

The PDE4 family of cAMP-specific phosphodiesterases represents an important therapeutic and pharmacological target for their anti-inflammatory and anti-depressant effects. Four distinct genes (PDE4A, PDE4B, PDE4C and PDE4D), encode large family of PDE4 isoforms. Almost all of these isoforms have a common core made up of central catalytic domain and C-terminal tail.

Research Area