GeneTex
United States (US)

PDE4A8 antibody

PDE4A8-antibody_ICCIF_GTX14606-1_18121411_883.png
PDE4A8-antibody_WB_GTX14606-2_18121411_683.png

Cat No. GTX14606

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Mouse, Rat
APPLICATION

Application Note

ICC: Use at a dilution of 1/250. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/1000. Predicted molecular weight: 106 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

98 kDa. ( Note )

Specificity/Sensitivity

Does not cross react with other PDE4A variants including the novel 76 kDa testis-specific PDE4A protein. Does not cross react with PDE4B, C or D proteins.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Thimerosal / Merthiolate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

N-terminus PDE4A8 synthetic cyclic peptide.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphodiesterase 4A,Dpde2,Pde4,Pde46,Pde4A

Background

The PDE4A8 is a testis germ cell-specific PDE4A variant. There are two PDE4A variants identified in rat testis (PDE4A8 and an un-characterized 76 kDa PDE4A protein). Spermatogenesis is accompanied by expression of a varied repertoire of PDE4 enzymes at different stages of cell differentiation that presumably perform specialized functions. Confocal microscopic examination revealed the presence of PDE4A8 in primary and secondary spermatids but not in mature spermatozoa. PDE4A8 inhibition results in increased apoptosis of the germ cells accompanied with reduction in the number of mature spermatozoa.

Database

Research Area

DATA IMAGES
PDE4A8-antibody_ICCIF_GTX14606-1_18121411_883.png

GTX14606 ICC/IF Image

Confocal micrograph of rat testis.
A: Section labeled with antibody GTX14607
B: Section labeled with antibody GTX14611

PDE4A8-antibody_WB_GTX14606-2_18121411_683.png

GTX14606 WB Image

Western blot of PDE4A8 using GTX14606

REFERENCE