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PDE4B antibody, C-term

Anti-PDE4B antibody, C-term used in Western Blot (WB). GTX89403
Anti-PDE4B antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89403
Anti-PDE4B antibody, C-term used in Western Blot (WB). GTX89403

Cat. No. GTX89403

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all PDE4B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1μg/ml
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89403-PEP).

Product Note

This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1).

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DIDIATEDKSPVDT, from the C Terminus of the protein sequence according to NP_002591.2; NP_001032418.1; NP_001032416.1; NP_001032417.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 4B , DPDE4 , PDEIVB

Background

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-PDE4B antibody, C-term used in Western Blot (WB). GTX89403

GTX89403 WB Image

WB analysis of mouse brain lysate using GTX89403 PDE4B antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-PDE4B antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89403

GTX89403 IHC-P Image

IHC-P analysis of human prostate using GTX89403 PDE4B antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-PDE4B antibody, C-term used in Western Blot (WB). GTX89403

GTX89403 WB Image

WB analysis of HEK293 overexpressing human PDE4B (mock transfection in first lane) using GTX89403 PDE4B antibody, C-term.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399