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PDE4C antibody [N3C3]

Anti-PDE4C antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX106268
Anti-PDE4C antibody [N3C3] used in Western Blot (WB). GTX106268
Anti-PDE4C antibody [N3C3] used in Western Blot (WB). GTX106268

Cat. No. GTX106268

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PDE4C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDE4C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951143

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 4C , DPDE1 , PDE21

Background

Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. PDE4C is a class IV cAMP-specific PDE (summary by Milatovich et al., 1994 [PubMed 8009369]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PDE4C antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX106268

GTX106268 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endo mitral, using PDE4C(GTX106268) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PDE4C antibody [N3C3] used in Western Blot (WB). GTX106268

GTX106268 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE4C antibody [N3C3] (GTX106268) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE4C antibody [N3C3] used in Western Blot (WB). GTX106268

GTX106268 WB Image

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX106268 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169