GeneTex
United States (US)

PDE4D antibody [C3], C-term

GTX104652_39820_IHC_19011506_813.jpg
GTX104652_39820_IFA_19011506_721.jpg
GTX104652_39820_WB_19011506_612.jpg

Cat No. GTX104652

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

A431 , HeLa
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PDE4D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686M11213 antibody, DPDE3 antibody, FLJ97311 antibody, HSPDE4D antibody, PDE4DN2 antibody, STRK1 antibody, PDE4D antibody, "phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) antibody", cAMP-specific phosphodiesterase PDE4D6 antibody, "cAMP-specific 3',5'-cyclic phosphodiesterase 4D antibody", dunce-like phosphodiesterase E3 antibody, "phosphodiesterase 4D, cAMP-specific antibody"

Cellular Localization

Cytoplasm (By similarity) , Membrane (By similarity) , Cytoplasm , cytoskeleton (By similarity) , Centrosome (By similarity)

Background

The PDE4D gene is complex and has at least 9 different isoforms that encode functional proteins. These proteins degrade the second messenger cAMP, which is a key signal transduction molecule in multiple cell types, including vascular cells (Dominiczak and McBride, 2003 [PubMed 14517535]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX104652_39820_IHC_19011506_813.jpg

GTX104652 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D(GTX104652) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104652_39820_IFA_19011506_721.jpg

GTX104652 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D(GTX104652) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

GTX104652_39820_WB_19011506_612.jpg

GTX104652 WB Image

Sample (30 µg of whole cell lysate)
A: A431
B: HeLa
7.5% SDS PAGE
GTX104652 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169