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PDE4D antibody [C3], C-term

Anti-PDE4D antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104652
Anti-PDE4D antibody [C3], C-term used in Western Blot (WB). GTX104652
Anti-PDE4D antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104652

Cat. No. GTX104652

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PDE4D3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

A431 , HeLa

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PDE4D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951145

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 4D , ACRDYS2 , DPDE3 , HSPDE4D , PDE43 , PDE4DN2 , STRK1

Cellular Localization

Cytoplasm , Membrane , Cytoplasm , cytoskeleton , Centrosome

Background

The PDE4D gene is complex and has at least 9 different isoforms that encode functional proteins. These proteins degrade the second messenger cAMP, which is a key signal transduction molecule in multiple cell types, including vascular cells (Dominiczak and McBride, 2003 [PubMed 14517535]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PDE4D antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104652

GTX104652 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D(GTX104652) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PDE4D antibody [C3], C-term used in Western Blot (WB). GTX104652

GTX104652 WB Image

Sample (30 μg of whole cell lysate)
A: A431
B: HeLa
7.5% SDS PAGE
GTX104652 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE4D antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104652

GTX104652 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D(GTX104652) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169