APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| 1:150 |
| 1:10000 |
Not tested in other applications.
Calculated MW
Product Note
This antibody labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, and PDE4D5 variants. Does not cross react with PDE4A, PDE4B, PDE4D6, PDE4D7, PDE4D8, PDE4D9, OR PDE4C.
PROPERTIES
Form
Liquid
Buffer
Tris/Glycine, 0.5% BSA, 30% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.57 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
C-terminal cyclic peptide unique to all PDED subtypes variants.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphodiesterase 4D , ACRDYS2 , DPDE3 , HSPDE4D , PDE43 , PDE4DN2 , STRK1
Cellular Localization
Apical cell membrane , Cytoplasm , Membrane , Cytoplasm, cytoskeleton
Background
This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
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GTX14613 WB Image
WB analysis of phosphorylated PDE4D protein lysate using GTX14613 PDE4D antibody.
Dilution : 1:500
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GTX14613 WB Image
WB analysis of PDE4D recombinant protein using GTX14613 PDE4D antibody.
Dilution : 1:500
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GTX14613 WB Image
WB analysis of various PDE4D subtype specific positive control lysates using GTX14613 PDE4D antibody.
Dilution : 1:500
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GTX14613 WB Image
WB analysis of various PDE4D, PDE4B and PDE4A variant lysates using GTX14613 PDE4D antibody.
Dilution : 1:1,000
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GTX14613 IHC-P Image
IHC-P analysis of human ovarian medulla tissue using GTX14613 PDE4D antibody.
Dilution : 1:50
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GTX14613 IHC-Fr Image
IHC-Fr analysis of abdominal skeletal muscle tissue using GTX14613 PDE4D antibody.
Dilution : 1:200
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REFERENCE
There are currently no references for PDE4D antibody (GTX14613). Be the first to share your publications with this product.
REVIEW
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