GeneTex
United States (US)

PDE4D antibody

GTX14613_WB_1_19011519_779.jpg
GTX14613_WB_2_19011519_281.jpg
GTX14613_IHC_1_19011519_913.jpg
GTX14613_WB_3_19011519_670.jpg

Cat No. GTX14613

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P Assay dependent
IP 1:150
ELISA 1:10,000
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

GTX14613 labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, PDE4D5 variants. Does not cross react with PDE4A, PDE4B or PDE4C.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Thimerosal / Merthiolate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

C-terminal cyclic peptide unique to all PDE4D subtypes variants.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.

Research Area

DATA IMAGES
GTX14613_WB_1_19011519_779.jpg

GTX14613 WB Image

WB analysis of various PDE4D, PDE4B, and PDE4A variants using GTX14613 PDE4D antibody.
1:1000

GTX14613_WB_2_19011519_281.jpg

GTX14613 WB Image

WB analysis of various PDE4 recombinant protein using GTX14613 PDE4D antibody.
1:500

GTX14613_IHC_1_19011519_913.jpg

GTX14613 IHC Image

IHC analysis of abdominal skeletal muscle using GTX14613 PDE4D antibody.
1:200

GTX14613_WB_3_19011519_670.jpg

GTX14613 WB Image

WB analysis of various PDE4D subtype recombinant protein using GTX14613 PDE4D antibody.
1:500

Package List Price ($)
$ 359