GeneTex
United States (US)

PDE4D3 antibody

PDE4D3-antibody_WB_GTX14614-1_18121411_141.jpg

Cat No. GTX14614

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

ICC: Use at an assay dependent dilution. IP: Use at a dilution of 1/150. WB: Use at a dilution of 1/500. Detects a band of approximately 93 kDa. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Rat cortical membranes

Specificity/Sensitivity

GTX14614 does not cross react with PDE4D1, D2, D4 and D5. Does not cross react with PDE4A, PDE4B or PDE4C.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide from unique region near N-terminal of the PDE4D3 variant.

Purification

Immunogen affinity purified
IgG concentration is 0.2mg/ml

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.

Research Area

DATA IMAGES
PDE4D3-antibody_WB_GTX14614-1_18121411_141.jpg

GTX14614 WB Image

Western blot of GTX14614 variant-selective antibody. Antibody dilution 1:500

REFERENCE
Package List Price ($)
$ 359