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PDE5A antibody, C-term

Anti-PDE5A antibody, C-term used in Western Blot (WB). GTX89598
Anti-PDE5A antibody, C-term used in Western Blot (WB). GTX89598

Cat. No. GTX89598

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all PDE5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89598-PEP).

Product Note

This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223).

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 5A , CGB-PDE , CN5A , PDE5

Background

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PDE5A antibody, C-term used in Western Blot (WB). GTX89598

GTX89598 WB Image

WB analysis of human lung lysate using GTX89598 PDE5A antibody, C-term.
Dilution : 0.3μg/ml
Loading : 30μg protein in RIPA buffer

Anti-PDE5A antibody, C-term used in Western Blot (WB). GTX89598

GTX89598 WB Image

WB analysis of HEK293 overexpressing PDE5A (mock transfection in first lane) using GTX89598 PDE5A antibody, C-term.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399