GeneTex
United States (US)

PDE5A antibody

PDE5A-antibody_ICCIF_GTX14672-1_18121411_204.jpg
PDE5A-antibody_WB_GTX14672-1_18121411_313.jpg

Cat No. GTX14672

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects bands of approximately 99 and 89 kDa. (predicted molecular weight: 105 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

95 kDa. ( Note )

Positive Control

Rat lung homogenate

Specificity/Sensitivity

This antibody detects 99 and 89 kDa PDE5A proteins in various tissues.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic cyclic peptide (common to all PDE5A variants) (Rat).

Purification

Immunogen affinity purified
Purified on antigen sepharose affinity column.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphodiesterase 5A,Pde5A2,Pde5A

Cellular Localization

Located mostly to soluble cellular fractions, and sometimes to particulate cellular fractions.

Background

The cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A. The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB PDEs). The cGB PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site.

Database

Research Area

DATA IMAGES
PDE5A-antibody_ICCIF_GTX14672-1_18121411_204.jpg

GTX14672 ICC/IF Image

Immunostaining of cells expressing PDE5 with GTX14672 antibody

PDE5A-antibody_WB_GTX14672-1_18121411_313.jpg

GTX14672 WB Image

Western blot of human recombinant GFP-PDE5A with PDE5A (GTX14672) and Phospho-PDE5A antibodies. Overexpressing cells were treated with or with out ANP. Antibody dilution 1:500 in DiluObuffer. The blot was stripped with StripObuffer and re-probed with Phospho-PDE5A antibody

REFERENCE