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PDE5A antibody

Anti-PDE5A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14672
Anti-PDE5A antibody used in Western Blot (WB). GTX14672

Cat. No. GTX14672

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μg ($409)
See all PDE5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:250
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:250
ELISA 1:10000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Product Note

This antibody detects 99 and 89 kDa PDE5A proteins in various tissues. This antibody does not cross-react with other PDE family members.

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine, 0.5% BSA, 30% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55-0.60 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_369424

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 5A , CGB-PDE , CN5A , PDE5

Background

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PDE5A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14672

GTX14672 ICC/IF Image

ICC/IF analysis of cells expressing PDE5 using GTX14672 PDE5A antibody.
Dilution : 1:200

Anti-PDE5A antibody used in Western Blot (WB). GTX14672

GTX14672 WB Image

WB analysis of recombinant GFP-PDESA1 over-expressed cells treated with or without Atrial natriuretic peptide (ANP) using GTX14672 PDE5A antibody or GTX36930 PDE5A (phospho Ser 92) antibody.
Dilution : 1:500

REFERENCE

Application Reference

Tinsley HN et al. Mol Cancer Ther 2009; 8 (12) : 3331-40 * sulfide selectively inhibits growth and induces apoptosis of human breast tumor cells by phosphodiesterase 5 inhibition, elevation of cyclic gmp, and activation of protein kinase g.
Application : WB
Reactivity : Human
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Package List Price ($)
$ 409