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PDE5A (phospho Ser 92) antibody

Anti-PDE5A (phospho Ser 92) antibody used in Western Blot (WB). GTX36930

Cat. No. GTX36930

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Zebrafish, Sheep, Bovine, Pig
Package
50 μl ($399)
See all PDE5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IP 1:200
ELISA 1:10000
IHC 1:100
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Product Note

This antibody will detect only PDE5A protein that has been phosphorylated at the PKG site. Will not detect unphosphorylated PDE5A, or other PDE family members.

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine, 0.5% BSA, 30% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0-1.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein. Phosphoryalted

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_11162077

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 5A , CGB-PDE , CN5A , PDE5

Background

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PDE5A (phospho Ser 92) antibody used in Western Blot (WB). GTX36930

GTX36930 WB Image

WB analysis of recombinant GFP-PDESA1 over-expressed cells treated with or without Atrial natriuretic peptide (ANP) using GTX14672 PDE5A antibody or GTX36930 PDE5A (phospho Ser 92) antibody.
Dilution : 1:500

REFERENCE

Application Reference

Mukohda M et al. J Clin Invest 2019; 129 (6) : 2318-2332 RhoBTB1 protects against hypertension and arterial stiffness by restraining phosphodiesterase 5 activity.
Li J et al. Gen Comp Endocrinol 2019; 286 : 113303 The role of PDE5a in oocyte maturation of zebrafish.
Application : WB
Reactivity : Zebrafish
Ale-Agha N et al. PLoS Biol 2018; 16 (6) : e2004408 CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine.
Application : WB
Reactivity : Human
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Package List Price ($)
$ 399