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PDE6D antibody [HL1359]

Recombinant antibody
Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799
Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799
Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799
Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799
Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799
Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799
Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799
Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799
Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799
Anti-PDE6D antibody [HL1359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636799

Cat. No. GTX636799

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1359

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all PDE6D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human PDE6D Recombinant protein

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910076

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 6D , JBTS22 , PDED

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799

GTX636799 WB Image

Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799

GTX636799 IHC-P Image

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat colon.
PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799

GTX636799 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799

GTX636799 IHC-P Image

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799

GTX636799 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799

GTX636799 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799

GTX636799 IHC-P Image

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDE6D antibody [HL1359] used in IHC (Paraffin sections) (IHC-P). GTX636799

GTX636799 IHC-P Image

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDE6D antibody [HL1359] used in Western Blot (WB). GTX636799

GTX636799 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (GTX636799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE6D antibody [HL1359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636799

GTX636799 ICC/IF Image

PDE6D antibody [HL1359] detects PDE6D protein at nucleus by immunofluorescent analysis.
Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDE6D stained by PDE6D antibody [HL1359] (GTX636799) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations