Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [N1C3] (GTX109240) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PDE6D(GTX109240) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PDE6D antibody [N1C3] detects PDE6D protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDE6D stained by PDE6D antibody [N1C3] (GTX109240) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [N1C3] (GTX109240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Mol Neurodegener in 2018.PMID: 30257685