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PDE6D antibody [N1C3]

Anti-PDE6D antibody [N1C3] used in Western Blot (WB). GTX109240
Anti-PDE6D antibody [N1C3] used in Western Blot (WB). GTX109240
Anti-PDE6D antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109240
Anti-PDE6D antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109240

Cat No. GTX109240

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

mouse eye

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human PDE6D Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951147

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphodiesterase 6D , JBTS22 , PDED

Database

Research Area

DATA IMAGES

Anti-PDE6D antibody [N1C3] used in Western Blot (WB). GTX109240

GTX109240 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PDE6D antibody [N1C3] (GTX109240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE6D antibody [N1C3] used in Western Blot (WB). GTX109240

GTX109240 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [N1C3] (GTX109240) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDE6D antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109240

GTX109240 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PDE6D(GTX109240) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PDE6D antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109240

GTX109240 ICC/IF Image

PDE6D antibody [N1C3] detects PDE6D protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDE6D stained by PDE6D antibody [N1C3] (GTX109240) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

REFERENCE

REVIEW

PDE6D antibody [N1C3] Cat No. GTX109240

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Neurodegener in 2018.PMID: 30257685
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169