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PDE6D antibody

Anti-PDE6D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25665
Anti-PDE6D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25665

Cat. No. GTX25665

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Sheep, Bovine
Package
100 μg ($399)
See all PDE6D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2.0 μg/ml
ICC/IF 1:10-1:200
IHC-Fr 1.0 μg/ml
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic Peptides: M(1) S A K D E R A R (D/E) I L R G F K(16) C

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphodiesterase 6D , JBTS22 , PDED

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-PDE6D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25665

GTX25665 ICC/IF Image

ICC/IF analysis of B-3 cells using GTX25665 PDE6D antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-PDE6D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25665

GTX25665 ICC/IF Image

ICC/IF analysis of BAEC cells using GTX25665 PDE6D antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399