GeneTex
United States (US)

PDE8A antibody

PDE8A-antibody_WB_GTX14618-1_18121411_782.jpg

Cat No. GTX14618

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 98-102 kDa. Optimal dilutions/concentrations should be determined by the end user.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Thimerosal / Merthiolate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic cyclic peptide from near the N terminus (common to all PDE8A variants).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP into AMP is phosphodiesterase type 8. The cAMP-specific phosphodiesterase type 8 (PDE8) family is comprised of 2 genes (PDE8A and PDE8B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. PDE8 family is a high affinity cAMP-specific, IBMX sensitive PDE. The PDE8 has a significant conserved region of about 270 amino acids common to all PDEs at the carboxy terminal apparently serves as the catalytic domain. The amino-terminal region of this protein is divergent and presumably accounts for the distinctive and regulatory properties unique to the individual PDE families. PDE8A protein showed significant homology to other cAMP-dependent PDEs (23%) within the catalytic domain. PDE8A is widely expressed in various tissues in contrast to PDE8B that is exclusively expressed in thyroid gland. The PDE8A transcripts are found in brain, pancreas, placenta, thyroid, spleen, trachea, prostate, and uterus.
DATA IMAGES
PDE8A-antibody_WB_GTX14618-1_18121411_782.jpg

GTX14618 WB Image

Western blot using GTX14618 antibody

Package List Price ($)
$ 319