FuncS: Use at an assay dependent dilution. This antibody is ideal for activity measurement and screening of various PDE8B enzyme variants in sample. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 98-100 kDa. Optimal dilutions/concentrations should be determined by the end user.
Rat testis and heart
This antibody has no cross-reactivity against PDE8A protein or any other PDE family members.
'Stabilization buffer' with 0.02% thimerosal or merthiolate.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide (Human).
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 5-prime,3-prime-cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been grouped into several families based on substrate affinities, inhibitor sensitivities, mode of regulation, and amino acid sequence homologies. The PDE8 family contains high-affinity cAMP-specific, IBMX (3-isobutyl-1-methyl-xanthine)-insensitive PDEs. All PDEs share a conserved C-terminal catalytic region and a variable N-terminal domain that presumably accounts for the distinctive regulatory properties unique to the individual families. PDE8B exhibits cAMP PDE activity which was not inhibited by various PDE inhibitors, including IBMX. The cAMP hydrolytic activity was unaffected by cGMP, and no cGMP PDE hydrolysis has been detected. Northern blot analysis indicated that the 4.2-kb PDE8B mRNA was expressed at the highest levels in thyroid, and at lower levels in brain, spinal cord, and placenta.
GTX14621 WB Image
Western blot of rat testis and heart homogenate using GTX14621 (1:500 dilution)