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PDGF Receptor alpha antibody [JF104-6]

Recombinant antibody
Anti-PDGF Receptor alpha antibody [JF104-6] used in Western Blot (WB). GTX01102
Anti-PDGF Receptor alpha antibody [JF104-6] used in Western Blot (WB). GTX01102
Anti-PDGF Receptor alpha antibody [JF104-6] used in IHC (Paraffin sections) (IHC-P). GTX01102
Anti-PDGF Receptor alpha antibody [JF104-6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01102
Anti-PDGF Receptor alpha antibody [JF104-6] used in Flow cytometry (FACS). GTX01102
Anti-PDGF Receptor alpha antibody [JF104-6] used in IHC (Paraffin sections) (IHC-P). GTX01102
Anti-PDGF Receptor alpha antibody [JF104-6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01102
Anti-PDGF Receptor alpha antibody [JF104-6] used in IHC (Paraffin sections) (IHC-P). GTX01102

Cat No. GTX01102

Host

Rabbit

Clonality

Monoclonal

Clone Name

JF104-6

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Observed MW

180-190 kDa. The observed M.W. is based on the following publication. PMID: 28403202

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

platelet derived growth factor receptor alpha , CD140A , GAS9 , PDGFR2 , PDGFRA , RHEPDGFRA , CD140a , PDGFR alpha , PDGF Receptor alpha

Cellular Localization

Cell membrane

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-PDGF Receptor alpha antibody [JF104-6] used in Western Blot (WB). GTX01102

GTX01102 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [JF104-6] (GTX01102) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication:PMID: 28403202

Anti-PDGF Receptor alpha antibody [JF104-6] used in Western Blot (WB). GTX01102

GTX01102 WB Image

Human tissue extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [JF104-6] (GTX01102) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDGF Receptor alpha antibody [JF104-6] used in IHC (Paraffin sections) (IHC-P). GTX01102

GTX01102 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01102 PDGF Receptor alpha antibody [JF104-6].

Anti-PDGF Receptor alpha antibody [JF104-6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01102

GTX01102 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX01102 PDGF Receptor alpha antibody [JF104-6].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-PDGF Receptor alpha antibody [JF104-6] used in Flow cytometry (FACS). GTX01102

GTX01102 FACS Image

FACS analysis of HeLa cells using GTX01102 PDGF Receptor alpha antibody [JF104-6].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-PDGF Receptor alpha antibody [JF104-6] used in IHC (Paraffin sections) (IHC-P). GTX01102

GTX01102 IHC-P Image

IHC-P analysis of mouse uterus tissue section using GTX01102 PDGF Receptor alpha antibody [JF104-6].

Anti-PDGF Receptor alpha antibody [JF104-6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01102

GTX01102 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX01102 PDGF Receptor alpha antibody [JF104-6].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-PDGF Receptor alpha antibody [JF104-6] used in IHC (Paraffin sections) (IHC-P). GTX01102

GTX01102 IHC-P Image

IHC-P analysis of mouse colon tissue section using GTX01102 PDGF Receptor alpha antibody [JF104-6].

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf