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PDGF Receptor alpha antibody [N2C2], Internal

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107903
Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903
Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903

Cat No. GTX107903

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

K562 , PC-12

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDGF Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951151

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

platelet derived growth factor receptor alpha , CD140A , PDGFR-2 , PDGFR2

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107903

GTX107903 ICC/IF Image

PDGF Receptor alpha antibody [N2C2], Internal detects PDGF Receptor alpha protein at cytoplasm by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDGF Receptor alpha stained by PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903

GTX107903 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:500.

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903

GTX107903 WB Image

Sample (30 ug of whole cell lysate)

A: K562

5% SDS PAGE

GTX107903 diluted at 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf