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PDGF Receptor alpha antibody [N2C2], Internal

Orthogonal
Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903
Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903
Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903
Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903
Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107903
Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903

Cat. No. GTX107903

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PDGF Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

K562 , PC-12

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDGF Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951151

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet derived growth factor receptor alpha , CD140A , PDGFR-2 , PDGFR2

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903

GTX107903 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903

GTX107903 WB Image

Human tissue extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903

GTX107903 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:500.

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903

GTX107903 WB Image

Sample (30 ug of whole cell lysate)

A: K562

5% SDS PAGE

GTX107903 diluted at 1:1000

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107903

GTX107903 ICC/IF Image

PDGF Receptor alpha antibody [N2C2], Internal detects PDGF Receptor alpha protein at cytoplasm by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDGF Receptor alpha stained by PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903

GTX107903 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Chen WT et al. Oncogene 2013; GRP78 as a regulator of liver steatosis and cancer progression mediated by loss of the tumor suppressor PTEN.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169