GeneTex
United States (US)

PDGF Receptor alpha antibody [N2C2], Internal

GTX107903_39988_20160121_WB_R_19011507_396.jpg
GTX107903_39988_WB_19011507_597.jpg

Cat No. GTX107903

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

K562 , PC-12

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDGF Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Platelet Derived Growth Factor Receptor Alpha,Cd140A,Pdgfr-2,Pdgfr2,Pdgfra

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107903_39988_20160121_WB_R_19011507_396.jpg

GTX107903 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:500.

GTX107903_39988_WB_19011507_597.jpg

GTX107903 WB Image

Sample (30 ug of whole cell lysate)

A: K562

5% SDS PAGE

GTX107903 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169