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PDGF Receptor alpha antibody

Anti-PDGF Receptor alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133619
Anti-PDGF Receptor alpha antibody used in Western Blot (WB). GTX133619

Cat No. GTX133619

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

NIH3T3

Predict Reactivity

Human, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PDGF Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887055

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

platelet derived growth factor receptor alpha , CD140A , PDGFR-2 , PDGFR2

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PDGF Receptor alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133619

GTX133619 ICC/IF Image

PDGF Receptor alpha antibody detects PDGF Receptor alpha protein at cytoplasm by immunofluorescent analysis.
Sample: NIH3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDGF Receptor alpha stained by PDGF Receptor alpha antibody (GTX133619) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PDGF Receptor alpha antibody used in Western Blot (WB). GTX133619

GTX133619 WB Image

Whole cell extract (40 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody (GTX133619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf