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PDGF Receptor alpha antibody

Orthogonal
Anti-PDGF Receptor alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133620
Anti-PDGF Receptor alpha antibody used in Western Blot (WB). GTX133620
Anti-PDGF Receptor alpha antibody used in Western Blot (WB). GTX133620

Cat. No. GTX133620

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all PDGF Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

NIH3T3

Predict Reactivity

Human, Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human PDGF Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887056

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet derived growth factor receptor alpha , CD140A , PDGFR-2 , PDGFR2

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PDGF Receptor alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133620

GTX133620 ICC/IF Image

PDGF Receptor alpha antibody detects PDGF Receptor alpha protein at cytoplasm by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDGF Receptor alpha stained by PDGF Receptor alpha antibody (GTX133620) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-PDGF Receptor alpha antibody used in Western Blot (WB). GTX133620

GTX133620 WB Image

Whole cell extract (40 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody (GTX133620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDGF Receptor alpha antibody used in Western Blot (WB). GTX133620

GTX133620 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody (GTX133620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169